Question

Sort the following steps of gpcr signal propagation (you may not use all the steps):

a) dephosphorylation of gpcr
b) g protein dissociation to adenylate cyclase and gtp binds to the g protein
c) ligand binding to gpcr
d) g protein dissociation from gpcr and gdp binds to the g protein
e) synthesis of camp
f) activation of pka
g) g protein association to adenylate cyclase
h) g protein dissociation from gpcr and gtp binds to the g protein g

Answer 1

GPCR signal propagation follows the sequence

Ligand binding to GPCR (c) - This activates the receptor.

G protein dissociation from GPCR and GTP binds to the G protein (h)

G protein association to adenylate cyclase (g)

Synthesis of cAMP (e)

Activation of PKA (f)

Step-by-step explanation:

Sorting the steps of GPCR (G-protein-coupled receptor) signal propagation involves several sequential processes. Here is the correct order based on the given information:

1. Ligand binding to GPCR (c) - This activates the receptor.
2. G protein dissociation from GPCR and GTP binds to the G protein (h) - The GTP binding causes the alpha and By subunits to dissociate.
3. G protein association to adenylate cyclase (g) - This is when GTP-bound alpha subunit associates with the adenylate cyclase.
4. Synthesis of cAMP (e) - Adenylate cyclase catalyzes the formation of cAMP from ATP.
5. Activation of PKA (f) - cAMP activates Protein Kinase A, leading to a phosphorylation cascade.

These steps illustrate a typical signal transduction pathway for a GPCR, where the ultimate response to the extracellular signal through GPCRs leads to cellular changes via second messengers like cAMP.