Final answer:
Increasing the concentration of substrate will increase the rate of reaction only if the substrate is the rate-limiting factor and the temperature is optimal for enzyme activity.
option d is the correct
Step-by-step explanation:
Increasing the concentration of substrate will increase the rate of reaction only if the substrate is the rate-limiting factor (option b).
Initially, an increase in substrate concentration leads to an increase in the rate of an enzyme-catalyzed reaction. However, as the enzyme molecules become saturated with substrate, this increase in reaction rate levels off.
Therefore, if there is too much enzyme (option a) or the enzyme is the rate-limiting factor (option c), increasing the substrate concentration will not increase the rate of reaction. Additionally, the rate of an enzyme-catalyzed reaction is also influenced by the temperature (option d). It is important to ensure that the temperature is optimal for enzyme activity to increase the rate of reaction.