Final answer:
Computer analysis of RNA-Seq data before and after heat shock would likely reveal differential gene expression, with increased regulation of heat response genes and potential post-transcriptional modifications as adaptive responses.
Step-by-step explanation:
When RNA-Seq is performed before and after heat shock of bacterial cells, computer analysis would be expected to show differential gene expression, with some genes being upregulated in response to stress, while others may be downregulated. Heat shock typically leads to an increase in the expression of heat shock proteins, which help the cell cope with stress. Additionally, RNA-Seq may reveal post-transcriptional adjustments, such as alternative splicing or differences in mRNA stability, which are essential for the bacteria to survive and adapt to the heat shock.
Furthermore, genes related to reactive oxygen species defense mechanisms and basic metabolic processes may also be adjusted based on their roles in stress response and survival. Modulations in the transcriptomic profile would indicate the cellular adjustments on an RNA level due to the environmental change induced by the heat shock.