Final answer:
The asymmetry of the membrane is created and demonstrated through techniques such as freeze-fracture electron microscopy, cytochemical demonstrations, and biochemical assays with proteolytic enzymes to reveal different components on either side of the membrane.
Step-by-step explanation:
The asymmetry of the membrane is created through several methods of examination and experiments. One of the primary techniques is freeze-fracture electron microscopy, which involves the freezing of isolated membranes in water and chipping the ice to reveal the features of the interior and exterior surfaces of the membrane. This process shows pits and mounds on opposite sides of the fractured membranes, providing visual evidence of membrane asymmetry.
Additionally, experiments with cytochemistry demonstrate that only the outer surfaces of plasma membranes are sugary coated, confirming asymmetry. Another method is the biochemical demonstration of membrane asymmetry, where the treatment of cells with proteolytic enzymes followed by the extraction of membrane proteins revealed differing components in the membrane, further confirming its asymmetric nature.