Question

In comparing the behavior of a 10,000 and 45,000 molecular weight protein using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and gel filtration column chromatography, which of the following statements is most likely to be true. Assume other proteins are single polypeptide chains with no disulfide bonds.

a. The smaller protein will migrate (move) faster relative to the larger protein in SDS-PAGE but slower in gel filtration.
b. The smaller protein will migrate (move) slower relative to the larger protein in both SDS-PAGE and gel filtration
c. The smaller protein will migrate (move) faster relative to the larger protein in both SDS-PAGE and gel filtration
d. The smaller protein will migrate slower (move) relative to the larger protein in SDS-PAGE but faster in gel filtration

Answer 1

a. The smaller protein will migrate (move) faster relative to the larger protein in SDS-PAGE but slower in gel filtration.

Step-by-step explanation:

Gel filtration chromatography is a technique of chromatography in which particles facilitate separation based on molecular size. The larger protein come out of the column and form gel permeation chromatograph. The smaller proteins move faster to migrate from the column.