Question

Check to see that the objective lens is clicked into place. Make sure the light source is on. Adjust the diaphragm until a larger opening is under the stage. What should be done next?

1) Turn off the light source
2) Adjust the diaphragm until a smaller opening is under the stage
3) Remove the objective lens
4) Move the stage up

Answer 1

The next step after adjusting the diaphragm for more light is to move the stage up using the stage adjustment knobs. This action will allow you to focus on the specimen before further optimizing the light using the condenser lens and iris diaphragm adjustments.

Step-by-step explanation:

After ensuring the objective lens is clicked into place, the light source is on, and the diaphragm is adjusted for a larger opening, the next step in using a microscope is to move the stage up. This is done with the stage adjustment knobs. Moving the stage up allows you to bring the specimen into focus.

Once the image is roughly in focus using the coarse focusing knob, you can then refine the focus with the fine focusing knob, especially when working with higher magnifications such as the 40x or 100x objective lenses. It's also important to adjust the condenser lens to focus the light on the specimen and use the iris diaphragm to adjust the light intensity to create the best possible image contrast and clarity.