Final answer:
In the biochemical pathway described, Enzyme 1 (E1) catalyzes the reaction that converts substrate A to B, indicating that E1 specifically utilizes substrate A only.
Step-by-step explanation:
The question pertains to a biochemical pathway involving substrates A, B, C, and enzymes E1 and E2. The asked detail is what substrate enzyme 1 (E1) can utilize. Based on the understanding of enzymatic reactions and enzyme specificity, an enzyme will catalyze the reaction of a specific substrate at its active site. Since the pathway shows A converting to B with the help of enzyme E1, it is clear that E1 can utilize substrate A. Additionally, no information is provided to suggest that E1 can act on substrate B; hence, E1 cannot utilize B. Therefore, the correct answer is that Enzyme 1 (E1) can utilize A only.