Final answer:
To determine which two genes are being amplified in a PCR lab, one must identify the primers used, as they dictate the DNA segments targeted for amplification. Common gene targets might include the beta-globin gene or the albumin gene.
Step-by-step explanation:
In the laboratory setting, when dealing with PCR amplification, it is essential to know what specific genes we are attempting to amplify. The question at hand pertains to a particular lab setup, and without specific details about the genes being targeted, a universal answer cannot be provided. However, generally in a PCR setup, specific segments of DNA are targeted, which are defined by the primers that are used. Primers are short pieces of DNA that are complementary to the beginning and end of the target sequence, and they bind to those respective parts of the template DNA.
For instance, if we were studying genetic diseases, you might aim to amplify segments of DNA that could include genes like the beta-globin gene, which is involved in blood disorders, or the albumin gene, pertinent to liver function assessments. To achieve amplification, you will need two primers: one is a top primer that is complementary to the beginning of the double-stranded DNA sequence, and the other is complementary to the end. Through the PCR cycles, these primers will define the boundaries of the DNA segment multiplied in the process.
In summary, the exact genes being amplified would depend on the lab's objective, but the key takeaway here is that PCR is used to selectively increase the quantity of a specific DNA sequence using primers specific to the target genes.