Final answer:
Certified non-GMO DNA is necessary for PCR reactions to ensure reliable detection and analysis of GMO content in food and feed samples, serving as a crucial control to verify the absence of GMO-related sequences and to maintain the integrity of the results.
Step-by-step explanation:
It is important to set up a PCR reaction with DNA from certified non-GMO food because the purpose of PCR is to amplify specific DNA sequences for detailed analysis, and using DNA from non-GMO sources ensures that the reference material is not already genetically modified, which could interfere with accurate detection and analysis. Certified non-GMO DNA serves as a control to demonstrate that the PCR is functioning correctly and to prove the absence of GMO-related genetic sequences when testing food samples.
This is especially relevant when using PCR and microarray technology for the identification of genetically modified organisms (GMOs) in food and feed.
Using non-GMO DNA as a control also helps ensure that any detection of GMO sequences is indeed from the sample being tested and not from any contamination. This is vital for producing reliable and defensible data, whether in food safety testing, disease pathogen detection, or quality control in agricultural production. The need to preserve the integrity of the PCR analysis is critical for researchers, regulatory agencies, and consumers who rely on the accuracy of these tests for making informed decisions.