Final answer:
Boiling a food sample with the Instagene matrix serves to extract and purify the DNA by denaturing DNA-degrading enzymes and protecting the genomic DNA, which is necessary for accurate downstream DNA analysis for purposes such as GMO detection, pathogen identification, and food safety investigations.
Step-by-step explanation:
Purpose of Boiling Food Sample + Instagene Matrix
The purpose of boiling the food sample along with the Instagene matrix is to carry out DNA extraction and purification from the food sample being tested. The Instagene matrix acts as a chelating resin which binds divalent cations such as Mg2+ that are essential cofactors for DNA-degrading enzymes like nucleases. By boiling, cellular components including the nucleases are denatured, ensuring that the genomic DNA remains intact. This process is important for subsequent analyses such as polymerase chain reaction (PCR), agarose gel electrophoresis, or microarray technology which rely on intact DNA for the detection of genetically modified organisms (GMOs), pathogens, or for food safety investigations.
For example, in a food safety investigation, DNA can be purified from a food sample to determine if harmful bacteria such as Listeria are present. Boiling the sample in the presence of Instagene ensures the preservation of the DNA for accurate detection using culture methods or DNA-based tests.