Final answer:
The purpose of using near-infrared light and the imaging agent Indocyanine Green (ICG) in creating fluorescent images of tissue is to enhance contrast, visualize specific molecules or structures, measure blood flow, and detect abnormalities or diseases in the tissue.
Step-by-step explanation:
The purpose of using near-infrared (NIR) light and the imaging agent Indocyanine Green (ICG) in creating fluorescent images of tissue is to enhance the contrast of the tissue, visualize specific molecules or structures in the tissue, measure the blood flow in the tissue, and detect abnormalities or diseases in the tissue.
NIR light allows for deeper penetration into the tissue, making it easier to visualize specific molecules or structures. ICG is a fluorescent dye that is commonly used as an imaging agent because it emits near-infrared light when excited by the NIR light. This allows for enhanced contrast in the fluorescent images of the tissue, making it easier to detect abnormalities or diseases.