Final answer:
Ethylene oxide sterilization requires careful placement of biologicals in plastic bags or containers to allow gas penetration, using sterilized forceps, and ensuring safety due to its carcinogenic nature. Proper aeration must follow.
Step-by-step explanation:
Ethylene oxide (EtO) sterilization is used to sterilize medical and laboratory equipment that is sensitive to heat. Biologicals for EtO sterilization should be placed in a manner that allows EtO gas to penetrate effectively. Typically, items to be sterilized are placed within plastic bags or specialized containers that are then introduced into the EtO sterilizer. The process requires careful handling due to the carcinogenic and explosive nature of ethylene oxide. After the sterilization process, sufficient aeration time is critical to ensure all residual EtO gas has dissipated from the sterilized items and packaging.
When setting up for EtO sterilization, one should use sterilized forceps, often disinfected with 70% ethanol, to handle the items. This helps maintain the sterility of the equipment that will be exposed to the sterilant. Laboratorians and healthcare professionals need to follow strict aseptic techniques to ensure proper sterilization and safety protocols are maintained.