Question

The Ti plasmid is used as a vector to transfer DNA into which of the following?

1) viruses
2) bacteria
3) plant cells
4) animal cells

Answer 1

The Ti plasmid is used as a vector to transfer DNA into plant cells, where it enables the integration of desired genes into the plant's genome for the creation of transgenic plants.

Step-by-step explanation:

The Ti plasmid is used as a vector to transfer DNA primarily into plant cells. This plasmid system is derived from the bacterium Agrobacterium tumefaciens, which is known for causing tumors in plants. In the field of genetic engineering, the tumor-inducing genes within the Ti plasmid are removed, and researchers insert the desired DNA fragment for transfer into the plant's genome. This method allows for the production of transgenic plants, which contain genes that have been inserted using the Ti plasmid.

During the process:

1. The desired gene is cloned into the Ti plasmid.
2. The recombinant Ti plasmid is introduced into plant cells.
3. The gene of interest recombines into the plant cell's genome.

As such, the correct answer to the question is option 3) plant cells.