Answer:
Restriction enzymes, also known as restriction endonucleases, play a crucial role in the defense mechanisms of bacteria against viral infections. Here's how they help prevent viral infections:
1. Recognizing foreign DNA: Restriction enzymes are proteins that can identify and bind to specific DNA sequences. These sequences are often present in the DNA of viruses but not in the bacterial DNA. By recognizing these foreign DNA sequences, restriction enzymes can distinguish between viral DNA and the host bacterial DNA.
2. Cutting viral DNA: Once the restriction enzyme identifies the specific DNA sequence, it cleaves the viral DNA at specific points, resulting in the fragmentation of the viral genome. This process is known as restriction digestion. The enzyme cuts the DNA at specific recognition sites, which are usually palindromic sequences (sequences that read the same forward and backward).
3. Preventing viral replication: The cleavage of viral DNA by restriction enzymes hampers the ability of the virus to replicate within the bacterial host. The fragmented viral DNA becomes non-functional and unable to carry out its normal functions, such as hijacking the host's cellular machinery for replication.
4. Protecting bacterial DNA: Bacterial DNA is protected from being cleaved by the restriction enzymes through a mechanism called methylation. Bacteria add a methyl group to their own DNA at the recognition sites of the restriction enzymes. This modification prevents the restriction enzymes from cutting the bacterial DNA, ensuring that the host's genetic material remains intact.
By cutting and inactivating viral DNA while preserving the integrity of the bacterial DNA, restriction enzymes provide a defense mechanism that helps prevent viral infections in bacteria. This system is known as the restriction-modification system and is a vital component of the bacterial immune system against viral invaders.
Step-by-step explanation: