Final answer:
DNA is the only option listed that is not directly involved in translation; it is used during transcription, which comes before translation.
Step-by-step explanation:
The process of translation involves the conversion of genetic information from an mRNA template into a specific protein sequence. The key components involved in translation include the mRNA, tRNAs, ribosomes, and various enzymatic factors like aminoacyl-tRNA synthetase enzymes. However, DNA is not directly involved in this process; it remains within the nucleus (in eukaryotic cells) and acts as the template for RNA synthesis during the transcription stage, which precedes translation. Understanding these distinct phases elucidates the intricate machinery steering cellular processes, crucial in comprehending genetic information flow and protein synthesis intricacies within biological systems.