Final answer:
Fructose-1 phosphate is not an intermediate in the glycolytic pathway; the intermediate after fructose-6-phosphate involves its phosphorylation to fructose-1,6-bisphosphate, catalyzed by phosphofructokinase.
Step-by-step explanation:
No, fructose-1 phosphate is not an intermediate of the glycolytic pathway. The glycolytic pathway involves the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate, catalyzed by the enzyme phosphofructokinase.
During glycolysis, glucose is transformed into fructose-6-phosphate. This molecule is then phosphorylated at the 1-position by another molecule of ATP to form fructose-1,6-bisphosphate, which is a key intermediate in glycolysis. This reaction is considered a committed step due to its large negative free energy change, and it is regulated according to the ATP/ADP ratio in the cell.
In summary, fructose-1 phosphate does not appear in the glycolytic pathway; instead, the important intermediate after the investment of the second ATP molecule is fructose-1,6-bisphosphate.