152k views
3 votes
Pseudomonas fluorescens after decolorization. Select the microbiological process:

A) Gram staining
B) Fermentation
C) Oxidation
D) Photosynthesis

1 Answer

7 votes

Final answer:

The microbiological process involving Pseudomonas fluorescens after decolorization is Gram staining (Option A), whereby Gram's iodine and alcohol are used to differentiate between Gram-positive bacteria, which retain the crystal violet stain, and Gram-negative bacteria, which do not.

The correct answer is options A) Gram staining.

Step-by-step explanation:

Gram staining is the microbiological process that involves Pseudomonas fluorescens after decolorization. This process utilizes Gram's iodine as a mordant, forming a complex with the crystal violet stain.

Alcohol is then used as a decolorizing agent, dehydrating the peptidoglycan layer and allowing retention of the crystal violet stain in Gram-positive bacteria but not in Gram-negative bacteria. A counterstain like safranin is applied, resulting in gram-positive bacteria appearing purple and gram-negative bacteria, including Pseudomonas fluorescens, appearing pink or red.

The correct answer is options A) Gram staining.

User DannySlor
by
9.0k points
Welcome to QAmmunity.org, where you can ask questions and receive answers from other members of our community.