Final answer:
The microbiological process involving Pseudomonas fluorescens after decolorization is Gram staining (Option A), whereby Gram's iodine and alcohol are used to differentiate between Gram-positive bacteria, which retain the crystal violet stain, and Gram-negative bacteria, which do not.
The correct answer is options A) Gram staining.
Step-by-step explanation:
Gram staining is the microbiological process that involves Pseudomonas fluorescens after decolorization. This process utilizes Gram's iodine as a mordant, forming a complex with the crystal violet stain.
Alcohol is then used as a decolorizing agent, dehydrating the peptidoglycan layer and allowing retention of the crystal violet stain in Gram-positive bacteria but not in Gram-negative bacteria. A counterstain like safranin is applied, resulting in gram-positive bacteria appearing purple and gram-negative bacteria, including Pseudomonas fluorescens, appearing pink or red.
The correct answer is options A) Gram staining.