Question

APase is highly subject to degradation. To ensure its stability, it must be mixed with another protein that acts to slow its self-destruction. The protein used for this purpose is bovine serum albumin (BSA). To stabilize the APase, mix 1 ml of stock "unstable" APase with 4 ml of BSA. MIX THOROUGHLY - invert and shake the tube holding this new solution. Record the dilution factor _________.

introduced by this step: The stock APase concentration is 10 mg/ml. What is the final concentration of APase after dilution?

Answer 1

The final concentration of APase after mixing 1 ml of a 10 mg/ml stock solution with 4 ml of BSA is 2 mg/ml, calculated by dividing the stock concentration by the dilution factor of 5.

Step-by-step explanation:

To calculate the final concentration of APase after dilution, we use the dilution factor, which is the total volume of the new mixture divided by the volume of the stock solution added. In this question, 1 ml of APase is mixed with 4 ml of BSA, resulting in a total of 5 ml of solution. The dilution factor is therefore 5 ml / 1 ml = 5.

The stock APase concentration given is 10 mg/ml. When we apply the dilution factor, the final concentration is 10 mg/ml divided by the dilution factor of 5, which equals 2 mg/ml. This is the concentration of APase in the new mixture with BSA.