Final answer:
The Cherokee culture was not one of the four major cultures of Mesoamerica and South America, which included the Inca, Aztec, Olmec, and Maya.
Step-by-step explanation:
The culture that was not one of the four major cultures of Mesoamerica and South America is the Cherokee.
The four major cultures of Mesoamerica and South America were the Inca, Aztec, Olmec, and Maya. These civilizations made significant contributions to the development of advanced agricultural systems, architecture, writing systems, and complex social structures.
The Cherokee, on the other hand, were a Native American culture that inhabited the southeastern United States and were not part of the Mesoamerican or South American civilizations.