Question

In RNAi experiments, a researcher inserts a piece of double-stranded RNA that matches part of a gene's coding sequence into cells, an embryo, or an organism. An RNAi experiment asks:

A) What is the structure of the inserted RNA?
B) How many genes are affected by the inserted RNA?
C) How does the inserted RNA replicate within the cells?
D) How does the inserted RNA impact the targeted gene's expression or function?

Answer 1

RNA interference (RNAi) is a molecular technique used to study gene function by silencing specific genes. In an RNAi experiment, a double-stranded RNA that matches part of a gene's coding sequence is inserted into cells, embryos, or organisms. This inserted RNA binds to the target gene's mRNA, preventing its translation into protein.

Step-by-step explanation:

RNA interference (RNAi) is a molecular technique used to study gene function by silencing specific genes. In an RNAi experiment, a double-stranded RNA that matches part of a gene's coding sequence is inserted into cells, embryos, or organisms. This inserted RNA binds to the target gene's mRNA, preventing its translation into protein. As a result, the expression or function of the targeted gene is impacted.