Question

Let’s assume the linker dna averages 54 bp in length (as discussed in the lecture). How many molecules of h3 would you expect to find in a dna sample that is 23,000 bp in length

Answer 1

To determine the number of molecules of H3 in a 23,000 bp DNA sample, calculate the number of nucleosomes and the number of molecules of H3 in each nucleosome. There would be 230 molecules of H3 in the DNA sample.

Step-by-step explanation:

To determine how many molecules of H3 you would expect to find in a 23,000 bp DNA sample, we need to calculate the number of nucleosomes and the number of molecules of H3 in each nucleosome. The DNA sample consists of 23,000 base pairs, with each nucleosome containing 200 base pairs (146 base pairs wrapped around the core histones and 54 base pairs spacer DNA). So, the number of nucleosomes in the DNA sample is 23,000 base pairs / 200 base pairs per nucleosome = 115 nucleosomes. Each nucleosome contains two copies of H3 histone, so the total number of molecules of H3 in the DNA sample would be 115 nucleosomes x 2 H3 molecules per nucleosome = 230 molecules of H3.