Final answer:
Submerging the origin line in the eluent during a TLC experiment affects the separation and resolution of components, leading to inaccurate Rf values.
Step-by-step explanation:
If Submerged Sammy's origin line on his TLC plate was immersed in the eluent, it would have a significant impact on the TLC experiment results. The origin line is where the sample mixture is applied, and it should be above the level of the eluent to allow the solvent to move up the plate by capillary action, carrying the sample with it. If the origin line is submerged, the sample can dissolve into the eluent, leading to a loss of material before it can be separated and potentially causing the compounds to spread horizontally rather than moving up the plate vertically. This effect jeopardizes the resolution of the components and the accuracy of the Rf values (the ratio of the distance moved by a compound to the distance moved by the solvent front).