Question

An enzyme catalyzes a reaction with a Km of 8.00 mm and a Vmax of 3.10 mm⋅s−1. Calculate the reaction velocity, V0, for each substrate concentration.

Answer 1

The reaction velocity, V0, for each substrate concentration can be calculated using the Michaelis-Menten equation.

Step-by-step explanation:

The reaction velocity, V0, for each substrate concentration can be calculated using the Michaelis-Menten equation.

1. Use the formula V0 = (Vmax * [S]) / (Km + [S]), where V0 is the reaction velocity, Vmax is the maximum initial rate, Km is the Michaelis-Menten constant, and [S] is the substrate concentration.
2. Plug in the values from the question: Vmax = 3.10 mm⋅s−1 and Km = 8.00 mm.
3. Substitute different [S] values in the equation and calculate the corresponding V0 for each substrate concentration.

For example, if [S] = 10.00 mm, the calculation becomes V0 = (3.10 mm⋅s−1 * 10.00 mm) / (8.00 mm + 10.00 mm) = 1.55 mm⋅s−1.