Final answer:
The difference between bacterial and eukaryotic transcription is that eukaryotic cells use three different RNA polymerases for transcription, whereas bacteria use a single RNA polymerase.
Step-by-step explanation:
A difference between bacterial and eukaryotic transcription is that in eukaryotes, there are different RNA polymerases (I, II, and III). Each of these transcribes a different subset of genes with RNA polymerase I responsible for transcribing rRNA genes, RNA polymerase II for all protein-coding genes, and RNA polymerase III for tRNA and small RNA genes. In bacteria, a single RNA polymerase transcribes all kinds of RNAs. Eukaryotic transcription also involves complex promoter sequences and various transcription factors for the initiation, while bacteria often contain operons that transcribe multiple polypeptides within the same mRNA.