Question

Calculate the weight of the buffers you will use to make the buffers for part A. What is the context or question related to this buffer preparation?

Answer 1

To prepare the 0.2 M tris buffer, calculate concentrations and masses, and transfer to a labeled bottle.

Step-by-step explanation:

To prepare the 0.2 M tris buffer, you need to calculate the concentrations of [A] (tris) and [HA] (trisHCl) using the Henderson-Hasselbalch equation. From there, you can determine the masses needed to prepare the buffer. After measuring the masses, add them to a 100.00 ml volumetric flask and fill it to the mark with "Millipore" water. Transfer the stock buffer to a bottle labeled with the contents (0.2 M tris buffer), pH, your name, and date.