Final answer:
Immersion in toluene is not a step in the preparation of specimens for bright-field light microscopy; it is used in other types of microscopy such as STM.
Step-by-step explanation:
The step that is NOT involved in the preparation of specimens for bright-field light microscopy is D: immersion in toluene. Toluene is not commonly used in the preparation for bright-field light microscopy, but is rather a fixative used in other types of microscopy such as Scanning Tunneling Microscopy (STM). The steps typically involved in preparing samples for bright-field microscopy include: A: dehydration in a series of alcohols, B: application of a fixative that penetrates the cell membrane and maintains cell structure, C: embedding in paraffin, and E: initial rinse in water of the fresh specimen under study. These steps help in preserving and detailing the specimen for clear viewing under a light microscope.