Question

You are using genetic engineering to design a protein that needs to be synthesized in the cytoplasm and then localized in the nucleus of the cell. To accomplish this, you could add

A) a DNA sequence for a nuclear localization signal into the DNA that will be present in the mature protein.
B) the protein during mitosis, at a time when the nuclear membrane is dispersed.
C) a nucleotide or few nucleotides to the DNA sequence for the protein.
D) a series of specific polysaccharides to the carboxyterminal end of the protein.
E) inverted repeats to the DNA sequence for the protein.

Answer 1

The correct option is to add a DNA sequence for a nuclear localization signal into the DNA that will be present in the mature protein.

Step-by-step explanation:

The correct answer is A) a DNA sequence for a nuclear localization signal into the DNA that will be present in the mature protein. The nuclear localization signal (NLS) is a short amino acid sequence that acts as a signal to direct the protein to the nucleus. By adding the NLS sequence to the DNA, the protein will be synthesized in the cytoplasm but will then be able to enter and localize in the nucleus.

For example, if you are designing a protein that needs to perform a specific function inside the nucleus, such as regulating gene expression, it is important for that protein to be able to enter the nucleus. By adding the NLS sequence to the protein's DNA, you ensure that the protein will be localized in the nucleus where it can perform its function.