Question

Using genetic engineering techniques, you remove the sequences that code for the ribosome-binding sequences of the bacterial LacZ gene. The removal of the sequences will lead to ___________.

(a) more LacZ protein produced due to faster ribosome movement across the mRNA
(b) transcriptional repression resulting in fewer mRNA milecules produced from this gene
(c) a longer half life
(d) translational inhibition of the LacA mRNA.

Answer 1

Removing the ribosome-binding sequences of the bacterial LacZ gene results in translational inhibition of the LacZ mRNA, preventing efficient translation initiation and leading to decreased production of LacZ protein.

Step-by-step explanation:

Using genetic engineering techniques to remove the sequences that code for the ribosome-binding sequences of the bacterial LacZ gene would lead to translational inhibition of the LacZ mRNA. This is because ribosome-binding sequences, or Shine-Dalgarno sequences, are crucial for the initiation of translation in prokaryotes like bacteria. These sequences are recognized by the ribosome and are necessary for the proper positioning of the ribosome on the mRNA to start translation. Without these sequences, ribosomes would not be able to bind efficiently to the mRNA, leading to a decrease in the production of LacZ protein.