Question

Experiment 1 is the positive control, demonstrating that the region of DNA upstream of the gene for GFP results in a pattern of expression that we normally find for the LKP1 gene. Experiment 2 shows what happens when the sites for binding factors X,Y, and Z are removed. Which experiment above demonstrates that factor X alone is sufficient for expression of the gene in the kidney?

(a) experiment 3
(b) experiment 4
(c) experiment 5
(d) experiment 6

Answer 1

Without specific details on Experiments 3 to 6, it's not possible to determine which experiment shows factor X alone is sufficient for gene expression in the kidney. Factor X alone would be sufficient if only the binding sites for Y and Z are removed.

Step-by-step explanation:

The question refers to a series of experiments using green fluorescence protein (GFP) as a reporter gene to monitor the pattern of expression for another gene. A positive control demonstrates normal expression patterns, while subsequent experiments remove binding sites for transcription factors X, Y, and Z to assess their roles in gene expression. The experiment that shows factor X alone is sufficient for expression in the kidney would be the one where only the sites for factors Y and Z are removed, leaving X intact.

However, as the specific experiments labeled 3, 4, 5, or 6 are not detailed in the given information, a direct answer to which experiment demonstrates that factor X alone is sufficient cannot be provided. The information required to identify the correct experiment (3, 4, 5, or 6) is missing.