Question

Events at the replication fork

____ joins the complex
new primer is synthesized
___ ____ displaces primase and then loads B clamp into primer site, ATP-->ADP

Pol 3 collides with the previous okazaki fragment and releases contact with the B clamp

Pol 3 displaces the clamp and then elongates the RNA primer to make a new okazaki fragment

clamp loader binds another B clamp to prepare for the next round of initiation

Answer 1

At the replication fork, the DNA helix is opened up by helicase, forming replication forks. DNA polymerase III uses an RNA primer to synthesize the daughter DNA strand on both the leading and lagging strands. Primers are replaced by DNA, and the Okazaki fragments are joined together by DNA ligase.

Step-by-step explanation:

At the replication fork, the DNA helix is opened up by helicase, forming replication forks. Single-strand binding proteins bind to the single-stranded DNA to prevent re-formation of the helix. Topoisomerase breaks and reforms the DNA's phosphate backbone ahead of the replication fork to relieve supercoiling. Primase synthesizes an RNA primer that is complementary to the DNA strand.

DNA polymerase III uses this RNA primer to synthesize the daughter DNA strand. On the leading strand, DNA is synthesized continuously, while on the lagging strand, DNA is synthesized in short fragments called Okazaki fragments. DNA polymerase I replaces the RNA primer with DNA, and DNA ligase seals the gaps between the Okazaki fragments to join them into a single DNA molecule.