Question

Assembly stages for Pol III holoenzyme:

gamma delta is the clamp loader
the clamp loader is displaced by ____
B dimer binds to dsDNA and binds DNA pol to core

Answer 1

The assembly stages for Pol III holoenzyme in prokaryotes involve the displacement of the clamp loader by the B dimer and the binding of the B dimer to the dsDNA. The B dimer then recruits the DNA pol to the core enzyme.

Step-by-step explanation:

In prokaryotes, the polymerase core enzyme is composed of four polypeptide subunits: α, a, β, and β'. There is a fifth subunit, o, which is involved only in transcription initiation. The holoenzyme, which is comprised of all five subunits, helps with the initiation and elongation of transcription. The clamp loader, made up of the Pol III holoenzyme:gamma delta, is displaced by the B dimer, which binds to the double-stranded DNA and then recruits the DNA pol to the core.