Final answer:
The replisome is involved in the replication of DNA, with proteins like helicase, DNA polymerase III, and DNA ligase playing critical roles in unwinding the DNA, synthesizing the new strands, and joining Okazaki fragments, respectively.
Step-by-step explanation:
The replisome is a complex of proteins engaged in the replication of the newly synthesized DNA strand. This intricate process involves multiple steps beginning with the unwinding of DNA at the origin of replication by helicase, continued by the stabilization of the unwound strands with single-strand binding proteins, and the prevention of supercoiling ahead of the replication fork by topoisomerase. DNA polymerase III adds nucleotides to the RNA primer synthesized by primase, ultimately leading to the elongation of both the lagging and leading strands, where the lagging strand is synthesized in Okazaki fragments. These fragments are later joined to form a continuous strand through the action of DNA ligase, creating phosphodiester bonds to seal the gaps and ensure a complete and faithful replication of the DNA molecule.