Question

General DNA replication:

The primosome moves ____ the replication fork, periodically initiating synthesis of the lagging strand Okazaki fragments. It synthesizes in the anti-elongation direction. The primase for both phiX174 and E. coli oriC priming is ____.

Role of DnaB protein:
1) 5' to 3' ___ activity generates movement of the replication fork.
2) It activates _____.

Answer 1

The primosome moves in the opposite direction of the replication fork to initiate synthesis of Okazaki fragments. DnaB protein has helicase activity and activates primase.

Step-by-step explanation:

The DNA replication fork moves in a direction away from the replication fork, periodically initiating synthesis of the lagging strand Okazaki fragments. It synthesizes in the anti-elongation direction. The primase for both phiX174 and E. coli oriC priming is an enzyme called primase.

The DnaB protein has two roles in DNA replication:

1. Its 5' to 3' helicase activity generates movement of the replication fork.
2. It activates primase, which synthesizes an RNA primer to initiate DNA synthesis by DNA polymerase.