Final Answer:
Priming of replication on double-stranded DNA always requires:
- Primase
- RNA primer
- DNA polymerase
Step-by-step explanation:
The process of priming replication on double-stranded DNA involves several key components. Firstly, primase is responsible for synthesizing a short RNA primer complementary to the DNA template. This RNA primer provides the starting point for DNA synthesis.
Secondly, the RNA primer itself is crucial in initiating the formation of the new DNA strand during replication. Finally, DNA polymerase takes over to extend the RNA primer with DNA nucleotides, facilitating the elongation of the nascent DNA strand.
In the context of DNA replication, priming is an essential step, and the involvement of primase, RNA primer, and DNA polymerase ensures the accurate and efficient synthesis of new DNA strands.
The RNA primer provides the necessary 3'-OH group for DNA polymerase to add nucleotides and build the complementary strand. This process is fundamental in both prokaryotic and eukaryotic DNA replication. It is important to note that the exception mentioned regarding rolling circle replication highlights a specific case where the 3'-end of the cut site itself acts as a primer, bypassing the need for a separate RNA primer in that particular mechanism.
Understanding the sequence of events in DNA replication is vital in molecular biology, as it sheds light on the intricate machinery and enzymes involved in maintaining the integrity and fidelity of the genetic material during cell division.