Final answer:
Protease activity refers to the ability of protease enzymes to break down proteins. In the lab, it is common to use full-length DNA instead of small fragments, and Klenow is an enzyme that helps prevent further digestion of the DNA. For DNA sequencing, uniform random labeling is used to identify specific nucleotides in the DNA sequence.
Step-by-step explanation:
Protease activity refers to the ability of protease enzymes to break down proteins. Some sites on DNA are sensitive to protease activity, meaning that the protease enzyme can cleave the DNA at those specific sites. This cleavage separates two regions on the DNA that have different functions.
In the lab, when working with small DNA fragments, it is often not practical to use them directly. Instead, the full-length DNA is used along with an enzyme called Klenow. Klenow prevents any further digestion or degradation of the DNA fragments. It can also be used to end-label the DNA fragments for specific studies.
For DNA sequencing, a different approach is used. Instead of using the full-length DNA, the DNA fragments are labeled with a uniform random labeling method. This allows for the identification of specific nucleotides in the DNA sequence during the sequencing process.