Question

DNA Polymerases Have

Various Nuclease Activities
DNA polymerase I has a unique 5′-3′ exonuclease activity.
It can be combined with DNA synthesis to perform ___ ___

Answer 1

DNA Polymerases Have Various Nuclease Activities DNA polymerase I has a unique 5′-3′ exonuclease activity, it can be combined with DNA synthesis to perform remove RNA primers during DNA synthesis and replace them with newly synthesized DNA.

Step-by-step explanation:

DNA polymerase I, which is one of the three main types of DNA polymerases in prokaryotes, possesses a unique 5′-3′ exonuclease activity. This activity allows DNA polymerase I to remove RNA primers from the DNA and replace them with newly synthesized DNA. The exonuclease activity of DNA polymerase I works in combination with its DNA synthesis activity to perform efficient DNA replication.

During DNA replication, RNA primers are initially synthesized by an enzyme called primase. These primers serve as starting points for DNA synthesis. Once DNA synthesis is initiated, DNA polymerase I comes into action. It recognizes the RNA primer and uses its exonuclease activity to remove the primer in the 5' to 3' direction. Then, the polymerization activity of DNA polymerase I adds new DNA nucleotides in the 5' to 3' direction, replacing the removed RNA primer with DNA.

Overall, the unique 5′-3′ exonuclease activity of DNA polymerase I plays a crucial role in the replication process by ensuring the proper removal and replacement of RNA primers. This activity helps maintain the integrity and accuracy of DNA replication.