Question

For the identification of protein components involved in DNA synthesis using in vitro complementation systems, which of the following steps is accurate?

A) Combine extracts from mutant and wild-type strains and assay in vitro.
B) Isolate DNA from a conditional mutant and assay in vitro.
C) Subject the mutant strain to different temperatures and observe its growth characteristics.
D) Fractionate the wild-type extract and complement the mutant by adding back purified fractions.

Answer 1

The accurate step for identifying protein components in DNA synthesis with in vitro complementation systems is to combine mutant and wild-type extracts and assay in vitro.

Step-by-step explanation:

For the identification of protein components involved in DNA synthesis using in vitro complementation systems, the accurate step is: Combine extracts from mutant and wild-type strains and assay in vitro. This process allows for the identification of the deficient protein in the mutant extract by complementing it with proteins present in the wild type. Conducting a systematic elimination study, mutant and wild-type extracts can be mixed and the ability to perform DNA synthesis can be assayed, allowing researchers to pinpoint which specific components are required for the process. The complementary components from the wild-type extract can remediate the deficiency in the mutant extract, confirming their involvement in DNA synthesis.