Question

The Ames test is used to assess mutagenicity of a substance by:

A. using bacterial strains that had a mutation blocking histidine biosynthesis.
B. exposing the bacteria to various concentrations of potential mutagens.
C. tracking the number of cells that mutated to synthesize histidine.
D. A and B.
E. All of the above.

Answer 1

Option E). The Ames test is used to assess mutagenicity by using bacterial strains that had a mutation blocking histidine biosynthesis, exposing the bacteria to potential mutagens, and tracking the number of cells that mutated to synthesize histidine.

Step-by-step explanation:

The Ames test is used to assess mutagenicity of a substance by:

1. using bacterial strains that had a mutation blocking histidine biosynthesis
2. exposing the bacteria to various concentrations of potential mutagens
3. tracking the number of cells that mutated to synthesize histidine

Therefore, the correct answer is E. All of the above. The Ames test is a widely employed method that uses bacteria to test whether a given chemical can cause mutations in the DNA of the test organism. It is used to detect mutagenic and potentially carcinogenic chemicals.