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What is the difference in cutting between Msp1 and Hpa11?

a) Msp1 cuts DNA at specific sites, while Hpa11 cuts RNA.
b) Msp1 and Hpa11 both cut DNA at the same recognition sequence.
c) Msp1 recognizes and cuts different DNA sequences compared to Hpa11.
d) Msp1 and Hpa11 are identical in their cutting patterns.

User Drizin
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1 Answer

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Final answer:

Msp1 and Hpa11 are restriction enzymes that cut at different DNA sequences, which is essential for gene cloning and recombinant DNA technology. Msp1 has different recognition sites than Hpa11 and neither cuts RNA.

Step-by-step explanation:

The difference between Msp1 and Hpa11 is that Msp1 recognizes and cuts different DNA sequences compared to Hpa11. Both Msp1 and Hpa11 are restriction enzymes, which are proteins that cut DNA molecules at specific recognition sites, a process essential for gene cloning and recombinant DNA technology. Msp1 cuts at a different sequence than Hpa11, and unlike what might be suggested in alternative answer choices, neither of them cuts RNA, nor do they have identical cutting patterns.

It is also important to note that the process of cutting DNA is quite precise. For example, when a restriction enzyme like Msp1 or Hpa11 catalyzes hydrolysis at specific sites, it can generate sticky ends or blunt ends, which are the result of staggered cuts or straight cuts across the DNA molecules, respectively. These ends can be particularly useful when scientists are attempting to insert a piece of DNA into a plasmid vector for cloning.

User Brian
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