Question

In the context of genetic engineering, what does the protocol of transforming pGREEN into JM101 involve?

a) Isolating RNA
b) Cloning a gene into bacteria
c) Sequencing DNA
d) Creating a protein microarray

Answer 1

The transforming of pGREEN into JM101 involves cloning a gene into bacteria, where a gene of interest is isolated, inserted into a vector, and introduced into host bacteria for expression of the gene.

Step-by-step explanation:

In the context of genetic engineering, the protocol of transforming pGREEN into JM101 most likely involves cloning a gene into bacteria. This process typically includes the isolation of a gene of interest, insertion of that gene into a plasmid (such as pGREEN), and subsequent introduction of this recombinant DNA into a host organism, such as the E. coli strain JM101. The goal is to manipulate the E. coli bacteria to express the inserted gene, which can be a part of producing proteins, conducting research, or developing genetically modified organisms (GMOs).

The process of cloning begins with the preparation of complementary DNA (cDNA) from mRNA, which is then inserted into a vector like pGREEN. Once the recombinant DNA is created, it can be introduced into bacteria via a transformation process. This enables the bacteria to express the gene and potentially produce the corresponding protein in large quantities, a fundamental technique used in biotechnology.