Question

What are the first two steps of the RAG1/2 and HMG mediated V(D)J rearrangement?

A) Cleavage of hairpin structures, joining of coding ends
B) Recognition of recombination signal sequences, cleavage of recombination signal sequences
C) Binding of RAG1/2 to RSS, hairpin formation
D) Synapsis of recombination signal sequences, cleavage of hairpin structures

Answer 1

The first two steps of the RAG1/2 and HMG mediated V(D)J rearrangement are the recognition of recombination signal sequences and the cleavage of recombination signal sequences.

Step-by-step explanation:

The first two steps of the RAG1/2 and HMG mediated V(D)J rearrangement are:

1. Recognition of recombination signal sequences
2. Cleavage of recombination signal sequences

During V(D)J rearrangement, the RAG1/2 proteins bind to specific DNA sequences called recombination signal sequences (RSS) located adjacent to the V, D, and J gene segments. This recognition allows for the formation of a complex that cleaves the DNA at the RSS, resulting in the generation of coding ends. These coding ends will then go on to join together, leading to the formation of a diverse repertoire of antigen receptor genes.