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4.how did the hae iii restriction enzyme distinguish between the taster and nontaster allele? explain in terms of snps.

User Catzilla
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Answer: See Below

Step-by-step explanation:

I am not sure if there is a figure that is supposed to go with this question but I will explain the concept.

Resitriction enzymes such as HaeIII recogniize and cut at specific plaindromic sequences of DNA. I have attached the sequence where HaeIII cuts.

If this sequence is changed even by one base pair then the enzyme will not cut. SNPs stand for Single Nucleotide Polymorphisms and are a single base change in the genome. In this case I assume the taster and non-taster alleles are different by an SNP.

Restriction Fragment Length Polymorphism (RFLP) is the actual technique that identifies individuals with certain SNPs by cutting the gene and running it on a gel to see what fragments are created.

If a normal gene has the HaeIII restriction site then the enzyme will cut. If the mutant gene has an SNP at the restriction site then it will not cut.

4.how did the hae iii restriction enzyme distinguish between the taster and nontaster-example-1
User Art Vanderlay
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