Final answer:
A spill kit should be used under the laminar flow hood if the broken bottle contained Tryptic Soy Broth (TSB) or any other hazardous substances, to ensure safety and prevent contamination.
Step-by-step explanation:
If a bottle breaks under the laminar flow hood, a spill kit should be used if the bottle contained any hazardous or potentially infectious substances.
This would include a bottle of Tryptic Soy Broth (TSB), as it is used to promote organism growth and could contain bacteria that may be hazardous outside a controlled environment. The laminar flow hood is designed to prevent contamination and maintain a sterile work area, so any spill, particularly of a biological nature, needs to be dealt with promptly and properly using a spill kit to ensure safety and asepsis.
If a bottle breaks under the laminar flow hood, a spill kit should be used if the bottle contained bacteria that can grow in the sterile broth. When the neck of the flask is broken off, bacteria can reach the sterile broth and organism growth occurs. A spill kit is necessary to clean up and prevent the spread of bacteria.