Final answer:
The true statement regarding Roanoke Indians and their leader Wingina is that relations with the Roanoke colonists deteriorated, leading to Wingina's murder by Lane's men, and escalating conflicts including the accidental English attack on Croatoan, an allied tribe.
Step-by-step explanation:
The relations between the Roanoke colonists and the local Secotan tribe deteriorated due to the actions of Sir Ralph Lane and his men. Of particular note, the chief of the Secotan, Wingina, also called Pemisapan, was initially friendly towards the English, as evidenced by his involvement with John White's portraits. Unfortunately, this relationship soured and culminated in Wingina being murdered by Lane's forces. The backlash from this event was evident when one of the colonists, George Howe, was brutally killed by the Secotan, effectively signaling their anger and disapproval of the English presence and aggression.
Subsequent to these events, the colonists, under the leadership of John White, conducted a nighttime attack on a village they mistook for a Secotan settlement. In a tragic error, they attacked their previous allies, the Croatoan, causing unnecessary casualties. These incidents highlight the mistakes made by the English in their handling of native relations, which ultimately contributed to the failure of the Roanoke colony. The faded relations between the English and the Secotan, the murder of Chief Wingina, and the accidental attack on the Croatoan village all serve as particularly stark reminders of the importance of diplomacy and understanding in colonial ventures.