Question

Suppose you have a DNA fragment that you would like to insert into PSAP. What is the best method to accomplish this?

1) Transformation
2) Transduction
3) Conjugation
4) Electroporation

Answer 1

The best method to insert a DNA fragment into PSAP is electroporation as it creates temporary pores in the cell membrane with an electric pulse, allowing for the introduction of recombinant DNA into both prokaryotic and eukaryotic cells.

Step-by-step explanation:

To insert a DNA fragment into PSAP, the most effective method would be electroporation. This technique is well-suited for both prokaryotic and eukaryotic cells, especially when introducing recombinant DNA. Unlike transformation, which generally involves exposing bacteria to conditions (like CaCl₂ and heat shock) that make their membranes permeable to DNA, electroporation involves using an electrical pulse to create temporary pores in cell membranes. The intense electric field from electroporation generates a charge difference across the cell membrane, which helps draw the negatively charged DNA inside the cell. This method is particularly good for eukaryotic cells, which are not naturally competent to take up foreign DNA and have more robust cell membranes compared to bacterial cells. Therefore, for introducing a DNA fragment into PSAP, electroporation is the recommended method of genetic engineering.