Question

Arrange the steps for preparing monoclonal antibodies in the correct order, from first to last:

1.Harvesting and isolating the desired hybridoma cells.
2.Immunizing an animal (usually a mouse) with the target antigen.
3.Fusing B cells from the immunized animal with myeloma cells to create hybridoma cells.
4.Cloning and selecting the hybridoma cells that produce the desired monoclonal antibodies.
5.Culturing the hybridoma cells to produce a large quantity of monoclonal antibodies.
6.Testing and characterizing the monoclonal antibodies for specificity and functionality.
What is the correct chronological order for these steps in the preparation of monoclonal antibodies?

Answer 1

The correct sequence for the production of monoclonal antibodies involves immunization, cell fusion to create hybridomas, cloning and selection, harvesting, culturing, and testing of the monoclonal antibodies, respectively.

Step-by-step explanation:

The correct chronological order for preparing monoclonal antibodies (mAbs) is as follows:

1. Immunizing an animal (usually a mouse) with the target antigen.
2. Fusing B cells from the immunized animal with myeloma cells to create hybridoma cells.
3. Cloning and selecting the hybridoma cells that produce the desired monoclonal antibodies.
4. Harvesting and isolating the desired hybridoma cells.
5. Culturing the hybridoma cells to produce a large quantity of monoclonal antibodies.
6. Testing and characterizing the monoclonal antibodies for specificity and functionality.

The process of producing monoclonal antibodies involves several steps, beginning with the immunization of an animal with an antigen to stimulate an immune response. The subsequent fusing of B cells with myeloma cells creates hybridoma cells, which are then cloned and selected for their ability to produce the desired antibody. These selected cells are cultured, producing monoclonal antibodies that are eventually harvested, tested, and characterized for clinical or research purposes.