Question

What are the basic features of the miniTI vector plasmids?

a) Circular DNA molecules with antibiotic resistance genes
b) Linear DNA molecules with reporter genes
c) Recombinant DNA molecules with restriction sites
d) Plasmids with fluorescent markers

Answer 1

MiniTI vector plasmids are circular DNA molecules with features such as antibiotic resistance genes and a multiple cloning site (MCS) for the introduction of foreign DNA. (option a)

Step-by-step explanation:

The basic features of miniTI vector plasmids are that they are circular DNA molecules that can contain genes for antibiotic resistance. They are used as vectors for cloning because they can replicate independently within a bacterial cell. A critical feature of plasmids is the multiple cloning site (MCS), which is a short DNA sequence with multiple cutting sites for different restriction endonucleases. These enzymes recognize specific DNA sequences and can introduce staggered cuts that produce 'sticky ends', which are useful for ligating foreign DNA fragments into the plasmid. Plasmids might also have reporters for identifying recombinant DNA molecules. However, fluorescent markers are not typically basic features of miniTI vector plasmids.

The most accurate description of miniTI vector plasmids among the given options is 'circular DNA molecules with antibiotic resistance genes'.