Final answer:
The major limitation of using line transects is that they may produce unrepresentative biodiversity data due to insufficient spatial coverage and difficulties in thoroughly sampling species-rich or vast areas, leading to potential data collection biases.
Step-by-step explanation:
One limitation of using a line transect to collect data is that it can yield unrepresentative or superficial biodiversity data, particularly in expansive or species-rich areas. Line transects may not cover enough area or the full range of species present, leading to potential biases in the data collection process. Especially in complex environments like the Isoso, it is difficult to achieve precise and detailed population density estimates with basic tools and non-professional personnel. Additionally, working in large study areas with mobile guide lines and noting their effectiveness despite being time consuming further highlights that line transects require significant time, expertise, and meticulous planning, which can be seen as a limitation in terms of efficiency and return on investment.