228k views
4 votes
Which of the following techniques is used to examine proteins?

A Northern blot
B Southern blot
C Western blot
D Microarray
E All of the above

User Yet
by
9.1k points

1 Answer

2 votes

Final answer:

Western blotting is the technique used to examine proteins, where proteins are separated by gel electrophoresis, transferred to a membrane, and detected using specific antibodies.

Step-by-step explanation:

The technique used to examine proteins is Western blotting. In a Western blot, proteins are separated by gel electrophoresis, transferred onto a membrane (typically nitrocellulose or PVDF), and detected using antibodies that are specific to the protein of interest. This method is valuable for identifying specific proteins in a mixture and can be used to assess protein size, quantify protein amounts, and detect post-translational modifications.

Southern blotting is used to find specific sequences in a DNA sample by separating DNA fragments on a gel, transferring them to a nylon membrane, and incubating with a DNA probe. Northern blotting is comparable to Southern blotting but is applied to RNA to detect gene expression. A microarray is another technique that can probe for many genetic sequences at once but does not directly examine proteins.

User Alex Borodin
by
8.8k points
Welcome to QAmmunity.org, where you can ask questions and receive answers from other members of our community.