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Which of the following techniques is used to examine proteins?

A Northern blot
B Southern blot
C Western blot
D Microarray
E All of the above

User Yet
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1 Answer

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Final answer:

Western blotting is the technique used to examine proteins, where proteins are separated by gel electrophoresis, transferred to a membrane, and detected using specific antibodies.

Step-by-step explanation:

The technique used to examine proteins is Western blotting. In a Western blot, proteins are separated by gel electrophoresis, transferred onto a membrane (typically nitrocellulose or PVDF), and detected using antibodies that are specific to the protein of interest. This method is valuable for identifying specific proteins in a mixture and can be used to assess protein size, quantify protein amounts, and detect post-translational modifications.

Southern blotting is used to find specific sequences in a DNA sample by separating DNA fragments on a gel, transferring them to a nylon membrane, and incubating with a DNA probe. Northern blotting is comparable to Southern blotting but is applied to RNA to detect gene expression. A microarray is another technique that can probe for many genetic sequences at once but does not directly examine proteins.

User Alex Borodin
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